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penta mutant sortase a esrta  (Addgene inc)


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    Structured Review

    Addgene inc penta mutant sortase a esrta
    Penta Mutant Sortase A Esrta, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+pet29+esrta/sortase+A+pentamutant+(eSrtA)+in+pET29+(Plasmid+%2375144)/bio_rxiv__64898__2026__03__09__710718-213-2-11
    Average 94 stars, based on 69 article reviews
    penta mutant sortase a esrta - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Convenient Auto-Processing Vector Based on Bamboo Mosaic Virus for Presentation of Antigens Through Enzymatic Coupling
    Article Snippet: .. For production of SrtA, the plasmid pET29-eSrtA was purchased from Addgene (Watertown, MA, USA). ..

    Article Title: Site-specific, kinetically inert conjugation of labels and/or carriers to target molecules such as his-tagged proteins via metal complex reagents
    Article Snippet: Visualization of bands was performed with an Instant Blue Coomassie stain (Expedion; ISB1L). .. Purification of His-Sortase The enzyme sortase A (SEQ ID NO: 16) was expressed in E. coli BL21(DE3) using the plasmid pET29_eSrtA (Addgene #75144) (Chen, Dorr et al. 2011) and purified via a Ni2+-NTA column as described in (Chen, Dorr et al. 2011). ..

    Purification:

    Article Title: Site-specific, kinetically inert conjugation of labels and/or carriers to target molecules such as his-tagged proteins via metal complex reagents
    Article Snippet: Visualization of bands was performed with an Instant Blue Coomassie stain (Expedion; ISB1L). .. Purification of His-Sortase The enzyme sortase A (SEQ ID NO: 16) was expressed in E. coli BL21(DE3) using the plasmid pET29_eSrtA (Addgene #75144) (Chen, Dorr et al. 2011) and purified via a Ni2+-NTA column as described in (Chen, Dorr et al. 2011). ..



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